Difference between revisions of "Part:BBa K2623007"
(11 intermediate revisions by 2 users not shown) | |||
Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K2623007 short</partinfo> | <partinfo>BBa_K2623007 short</partinfo> | ||
− | === | + | ===Summary=== |
Secreted heat soluble protein acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities. The secretory TDP protein of water bear is used to improve the resistance of the bacteria under the conditions such as dehydration.<br> | Secreted heat soluble protein acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities. The secretory TDP protein of water bear is used to improve the resistance of the bacteria under the conditions such as dehydration.<br> | ||
− | A separate fragment is 510bp. | + | A separate fragment is 510bp<table><tr><th>[[Image:CAHS Fig1.png|thumb|300px|]]</th><th></table> |
+ | . | ||
+ | <br> | ||
+ | We transformed the plasmid to the E.coli(BL21), which is always used to express proteins with high efficience to verify the SAHS protein was produced successfully with a small scale.Besides, whether the SAHS protein with a signal peptide could be secreted was determined by SDS-PAGE. | ||
+ | <table><tr><th>[[Image:SAHS pro Gel 1.png|thumb|800px|Fig.1 The marker is on the lest, followed by our control group ( the BL21 with the empty plasmid), and the third well is the concentrated supernatant. The unit of the marker is "Kd". ]]</th><th></table> | ||
− | |||
− | |||
<br> | <br> | ||
+ | |||
+ | More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results <br> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 14:29, 15 October 2019
SAHS coding region
Summary
Secreted heat soluble protein acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities. The secretory TDP protein of water bear is used to improve the resistance of the bacteria under the conditions such as dehydration.
.
We transformed the plasmid to the E.coli(BL21), which is always used to express proteins with high efficience to verify the SAHS protein was produced successfully with a small scale.Besides, whether the SAHS protein with a signal peptide could be secreted was determined by SDS-PAGE.
More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 218
Illegal XhoI site found at 442 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 497
Illegal SapI.rc site found at 492