Difference between revisions of "Part:BBa K2623005"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2623005 short</partinfo>
 
<partinfo>BBa_K2623005 short</partinfo>
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===SUMMARY===
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===Summary===
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It is a component of the Kai circuit, which dephosphorylates the phosphorylated RpaA, preventing phosphorylated RpaA from continually activating transcription of related promoters.  
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It is a component of the Kai circuit, which dephosphorylates the phosphorylated RpaA, preventing phosphorylated RpaA from continually activating transcription of related promoters.
 
A separate fragment is 2265bp.
 
A separate fragment is 2265bp.
  
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===Structure of CikA===
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https://static.igem.org/mediawiki/parts/7/75/CikA.png
  
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===CikA's function in phosphorylation pathway===
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<br>
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<table><tr><th>[[Image:PKaiBC_RpaA_.gif|thumb|700px|Fig.1 Simplified model of the central circadian oscillator and output activities.[1]]]</th><th></table>
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===Reference===
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[1]Joseph S. Markson,Joseph R. Piechura,Anna M. Puszynska,Erin K. O’Shea. Circadian Control of Global Gene Expression by the Cyanobacterial Master Regulator RpaA[J]. Cell,2013,155(6).<br>
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More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results <br>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 19:56, 17 October 2018


CikA coding region

Summary

It is a component of the Kai circuit, which dephosphorylates the phosphorylated RpaA, preventing phosphorylated RpaA from continually activating transcription of related promoters. A separate fragment is 2265bp.

Structure of CikA

CikA.png

CikA's function in phosphorylation pathway


Fig.1 Simplified model of the central circadian oscillator and output activities.[1]

Reference

[1]Joseph S. Markson,Joseph R. Piechura,Anna M. Puszynska,Erin K. O’Shea. Circadian Control of Global Gene Expression by the Cyanobacterial Master Regulator RpaA[J]. Cell,2013,155(6).
More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 127
    Illegal BamHI site found at 2187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]