Difference between revisions of "Part:BBa K2623001"

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<partinfo>BBa_K2623001 short</partinfo>
 
<partinfo>BBa_K2623001 short</partinfo>
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===SUMMARY===
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===Summary===
 
KaiA is a component of the Kai circuit, which can active KaiC’s autophosphorylation.<br>
 
KaiA is a component of the Kai circuit, which can active KaiC’s autophosphorylation.<br>
 
Component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. In the complex, it enhances the phosphorylation status of KaiC. In contrast, the presence of KaiB in the complex decreases the phosphorylation status of KaiC, suggesting that KaiB acts by antagonizing the interaction between KaiA and KaiC. A KaiA dimer is sufficient to enhance KaiC hexamer phosphorylation.<br>
 
Component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. In the complex, it enhances the phosphorylation status of KaiC. In contrast, the presence of KaiB in the complex decreases the phosphorylation status of KaiC, suggesting that KaiB acts by antagonizing the interaction between KaiA and KaiC. A KaiA dimer is sufficient to enhance KaiC hexamer phosphorylation.<br>
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===Structure of KaiA===
 
===Structure of KaiA===
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[[Image:KaiA.png|thumb|500px|Fig.1]]
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<table><tr><th>[[Image:KaiA.png|thumb|500px|Fig.1 Protein structure of KaiA]]</th><th></table>
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===KaiA's function in KaiC's phosphorylation stage===
 
===KaiA's function in KaiC's phosphorylation stage===
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[[Image:Kai_1_.gif|thumb|900px|Fig.2]]
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<table><tr><th>[[Image:Kai_1_.gif|thumb|900px|Fig.2 Simplified scheme of the central circadian oscillator system.[1]]]
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</th><th></table>
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===Reference===
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[1]Paddock, Mark, Boyd, et al. Active Output State of the Synechococcus Kai Circadian Oscillator[J]. Pnas, 2014, 110(40):E3849-E3857.
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More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results <br>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 19:50, 17 October 2018


KaiA coding region

Summary

KaiA is a component of the Kai circuit, which can active KaiC’s autophosphorylation.
Component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. In the complex, it enhances the phosphorylation status of KaiC. In contrast, the presence of KaiB in the complex decreases the phosphorylation status of KaiC, suggesting that KaiB acts by antagonizing the interaction between KaiA and KaiC. A KaiA dimer is sufficient to enhance KaiC hexamer phosphorylation.
A separate fragment is 852bp.

Structure of KaiA

Fig.1 Protein structure of KaiA


KaiA's function in KaiC's phosphorylation stage

Fig.2 Simplified scheme of the central circadian oscillator system.[1]

Reference

[1]Paddock, Mark, Boyd, et al. Active Output State of the Synechococcus Kai Circadian Oscillator[J]. Pnas, 2014, 110(40):E3849-E3857.

More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]