Difference between revisions of "Part:BBa K2549004:Design"

(→‎References)
m
 
(5 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2549004 short</partinfo>
 
<partinfo>BBa_K2549004 short</partinfo>
−
 
 
<partinfo>BBa_K2549004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2549004 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
−
None
+
LaG17<ref>A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60  PMID: 25362362; DOI: 10.1038/nmeth.3170</ref> is served as the extracellular domain of our SynNotch receptor, thus accomplishing contact-dependent signal input against GFP. This nanobody recognizes our surEGFP ([[Part:BBa_K2446051]]) expressing cells.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
−
 
+
From IDT (gBlock), codon optimized for human
−
IDT(gBlock)
+
  
 
===References===
 
===References===
−
[1]A robust pipeline for rapid production of versatile nanobody repertoires.
 
−
Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP.
 
−
Nat Methods, 2014 Dec;11(12):1253-60  PMID: 25362362; DOI: 10.1038/nmeth.3170
 

Latest revision as of 13:13, 12 October 2018


anti-GFP (LaG17)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 34


Design Notes

LaG17[1] is served as the extracellular domain of our SynNotch receptor, thus accomplishing contact-dependent signal input against GFP. This nanobody recognizes our surEGFP (Part:BBa_K2446051) expressing cells.

Source

From IDT (gBlock), codon optimized for human

References

  1. ↑ A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170