Difference between revisions of "Part:BBa K2548012:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides with a broad spectrum of antimicrobial activity. SpALF4 is an ALF homolog identified from Scylla paramamosain (Mud crab) that has shown antimicrobial activity against A. hydrophila. Our aim is to obtain the cDNA sequence of SpALF4 from Scylla paramamosain and insert it into the genetic expression of E. coli to express large amount of SpALF4 to treat A. hydrophila invasions. | ||
===Source=== | ===Source=== | ||
| − | Scylla paramamosain | + | Zhu, L., Lan, J., Huang, Y., Zhang, C., Zhou, J., Fang, W., Li, X. (2014). SpALF4: A newly identified anti-lipopolysaccharide factor from the mud crab Scylla paramamosain with broad-spectrum antimicrobial activity. Fish & Shellfish Immunology,36(1), 172-180. doi:10.1016/j.fsi.2013.10.023 |
===References=== | ===References=== | ||
Latest revision as of 14:14, 16 October 2018
LacI - pTac - SpALF4 + 6xHis Tag - Terminator
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides with a broad spectrum of antimicrobial activity. SpALF4 is an ALF homolog identified from Scylla paramamosain (Mud crab) that has shown antimicrobial activity against A. hydrophila. Our aim is to obtain the cDNA sequence of SpALF4 from Scylla paramamosain and insert it into the genetic expression of E. coli to express large amount of SpALF4 to treat A. hydrophila invasions.
Source
Zhu, L., Lan, J., Huang, Y., Zhang, C., Zhou, J., Fang, W., Li, X. (2014). SpALF4: A newly identified anti-lipopolysaccharide factor from the mud crab Scylla paramamosain with broad-spectrum antimicrobial activity. Fish & Shellfish Immunology,36(1), 172-180. doi:10.1016/j.fsi.2013.10.023