Difference between revisions of "Part:BBa K2548005"

 
 
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aiiA gene (no LVA tag)
 
aiiA gene (no LVA tag)
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<h2>Construction of Quorum Quenching Device II (BBa_K2548000 + BBa_K2548007)</h2>
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https://static.igem.org/mediawiki/parts/thumb/e/e6/Quorumquenchingdevice2.png/799px-Quorumquenchingdevice2.png
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  <h2>Characterization of Quorum Quenching Device II</h2>
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  [[Image:QQresults.png|thumb|center|400px|Fig.1 Quorum quenching results]]<br>
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  <p>We did the experiment XIX to test the quorum quenching ability of QQ Device I (BBa_K2548000 + BBa_K2548006) and QQ Device II (BBa_K2548000 + BBa_K2548007), and the result is shown below. E. coli DH5a transformed with sensor device I was induced for 3 hours in the supernatant of cells containing empty RPG plasmid, QQ device I, and QQ device II after they were induced by C4-HSL for 4 hours. In Fig. 1, the control group with empty RPG plasmid shows higher GFP concentration than the culture of QQ device I and QQ device II, indicating that C4-HSL was consumed by the two devices. The GFP concentrations reduced 18.5% and 20.9% for device I and device II compared with control group respectively, implying that QQ device I had better C4-HSL degradation ability.</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:24, 17 October 2018


aiiA Codon Optimized

aiiA gene (no LVA tag)

Construction of Quorum Quenching Device II (BBa_K2548000 + BBa_K2548007)

799px-Quorumquenchingdevice2.png

Characterization of Quorum Quenching Device II

Fig.1 Quorum quenching results

We did the experiment XIX to test the quorum quenching ability of QQ Device I (BBa_K2548000 + BBa_K2548006) and QQ Device II (BBa_K2548000 + BBa_K2548007), and the result is shown below. E. coli DH5a transformed with sensor device I was induced for 3 hours in the supernatant of cells containing empty RPG plasmid, QQ device I, and QQ device II after they were induced by C4-HSL for 4 hours. In Fig. 1, the control group with empty RPG plasmid shows higher GFP concentration than the culture of QQ device I and QQ device II, indicating that C4-HSL was consumed by the two devices. The GFP concentrations reduced 18.5% and 20.9% for device I and device II compared with control group respectively, implying that QQ device I had better C4-HSL degradation ability.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 93
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 27
    Illegal NgoMIV site found at 616
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 535