Difference between revisions of "Part:BBa K2548004"
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AHL-lactonase from Bacillus thuringiensis strain 147-11516 (Pedroza). | AHL-lactonase from Bacillus thuringiensis strain 147-11516 (Pedroza). | ||
| − | + | <h2>Construction of Quorum Quenching Device I (BBa_K2548000 + BBa_K2548006)</h2> | |
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| + | https://static.igem.org/mediawiki/parts/thumb/6/6e/Quorumquenchingdevice1.png/800px-Quorumquenchingdevice1.png | ||
| + | <h2>Characterization of Quorum Quenching Device I</h2> | ||
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| + | [[Image:QQresults.png|thumb|center|400px|Fig.1 Quorum quenching results]]<br> | ||
| + | <p>We did the experiment XIX to test the quorum quenching ability of QQ Device I (BBa_K2548000 + BBa_K2548006) and QQ Device II (BBa_K2548000 + BBa_K2548007), and the result is shown below. E. coli DH5a transformed with sensor device I was induced for 3 hours in the supernatant of cells containing empty RPG plasmid, QQ device I, and QQ device II after they were induced by C4-HSL for 4 hours. In Fig. 1, the control group with empty RPG plasmid shows higher GFP concentration than the culture of QQ device I and QQ device II, indicating that C4-HSL was consumed by the two devices. The GFP concentrations reduced 18.5% and 20.9% for device I and device II compared with control group respectively, implying that QQ device I had better C4-HSL degradation ability.</p> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 21:23, 17 October 2018
AHL-lactonase
AHL-lactonase from Bacillus thuringiensis strain 147-11516 (Pedroza).
Construction of Quorum Quenching Device I (BBa_K2548000 + BBa_K2548006)
Characterization of Quorum Quenching Device I
We did the experiment XIX to test the quorum quenching ability of QQ Device I (BBa_K2548000 + BBa_K2548006) and QQ Device II (BBa_K2548000 + BBa_K2548007), and the result is shown below. E. coli DH5a transformed with sensor device I was induced for 3 hours in the supernatant of cells containing empty RPG plasmid, QQ device I, and QQ device II after they were induced by C4-HSL for 4 hours. In Fig. 1, the control group with empty RPG plasmid shows higher GFP concentration than the culture of QQ device I and QQ device II, indicating that C4-HSL was consumed by the two devices. The GFP concentrations reduced 18.5% and 20.9% for device I and device II compared with control group respectively, implying that QQ device I had better C4-HSL degradation ability.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 31
Illegal NgoMIV site found at 616
Illegal AgeI site found at 151 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 121