Difference between revisions of "Part:BBa K2669000:Experience"

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
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===Applications of BBa_K2669000===
 
===Applications of BBa_K2669000===
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After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells.  Please note: The exact procedure can be found  <a href="http://2018.igem.org/wiki/index.php?title=Team:Uppsala/UnaG"><b>here</b></a> Bilirubin tests (addition of a small amount of bilirubin to samples) allowed us to see if the UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin.  </p>
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<h1>iGEM Uppsala 2018 Experience</h1>
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After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells.  <b>Please note:</b> The exact procedure can be found at the end of <a href="http://2018.igem.org/wiki/index.php?title=Team:Uppsala/UnaG"><b>our UnaG wiki page</b></a> or on the main page.  Conducting "bilirubin tests" (the addition of a small amount of bilirubin dissolved in chloroform to samples) allowed us to see if UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin.  </p>
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<p>The part seems to work as intended.  It has a histidine tag, expresses UnaG, and fluoresces when cloned into cells that are subsequently exposed to bilirubin </p>
  
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<img class="content-card-img" src="https://static.igem.org/mediawiki/2018/2/20/T--Uppsala--UnaG_Comparison.png" alt="UnaG Comparison">
 
  
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<p><b>Figure 2:</b> Bilirubin test before/after affinity chromatography.  Going from right to left the samples are:</p>
 
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                            <ul>
 
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                                <li> Lysed sample of the “bad” part before AC</li>
 
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                                <li> Lysed sample of the “good” part before AC</li>
 
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                                <li> "Bad" part after AC</li>
 
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                                <li> "Good" part after AC</li>
 
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===User Reviews===
 
===User Reviews===
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Latest revision as of 02:23, 18 October 2018

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.


Applications of BBa_K2669000

iGEM Uppsala 2018 Experience

After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells. Please note: The exact procedure can be found at the end of our UnaG wiki page or on the main page. Conducting "bilirubin tests" (the addition of a small amount of bilirubin dissolved in chloroform to samples) allowed us to see if UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin.

The part seems to work as intended. It has a histidine tag, expresses UnaG, and fluoresces when cloned into cells that are subsequently exposed to bilirubin

===User Reviews=== BBa_K2669000 StartReviews BBa_K2669000 EndReviews