Difference between revisions of "Part:BBa K2623003"
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<partinfo>BBa_K2623003 short</partinfo> | <partinfo>BBa_K2623003 short</partinfo> | ||
− | === | + | ===Summary=== |
It is the core part of the Kai circuit, acting as a hexamer. This part can be both auto phosphorylated and dephosphorylated, and it can achieve about 24 hours of phosphorylation-dephosphorylation oscillation under the regulation of KaiA and KaiB. <br> | It is the core part of the Kai circuit, acting as a hexamer. This part can be both auto phosphorylated and dephosphorylated, and it can achieve about 24 hours of phosphorylation-dephosphorylation oscillation under the regulation of KaiA and KaiB. <br> | ||
− | Each KaiC monomer has two subunits, N-terminal CI subunit and C-terminal CII subunit. Each CII subunit has 431 serine (S431) residue and 432 threonine (T432) residue phosphorylation sites. Therefore, KaiC monomer has four possible phosphorylation states, showing different functions. SasA proteins will affect downstream signaling pathways, when it is phosphorylated by KaiC hexamers in a specific phosphorylated state. | + | Each KaiC monomer has two subunits, N-terminal CI subunit and C-terminal CII subunit. Each CII subunit has 431 serine (S431) residue and 432 threonine (T432) residue phosphorylation sites(Fig3). Therefore, KaiC monomer has four possible phosphorylation states, showing different functions. SasA proteins will affect downstream signaling pathways, when it is phosphorylated by KaiC hexamers in a specific phosphorylated state. |
A separate fragment is 1560bp. | A separate fragment is 1560bp. | ||
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===Structure of KaiC=== | ===Structure of KaiC=== | ||
+ | <table><tr><th> | ||
+ | [[Image:KaiC_z.png|thumb|200px|Fig.1 Planform of KaiC]]</th><th> | ||
+ | [[Image:KaiC_x.png|thumb|200px|Fig.2 Lateral view of KaiC]]</th><th> | ||
+ | [[Image:KaiC_1_.gif|thumb|300px|Fig.3 Each KaiC monomer has two subunits, N-terminal CI subunit and C-terminal CII subunit. Each CII subunit has 431 serine (S431) residue and 432 threonine (T432) residue phosphorylation sites.[1]]]</th><th></table> | ||
− | [[Image: | + | ===KaiC's phosphorylation stage=== |
− | [[ | + | <table><tr><th>[[Image:Kai_1_.gif|thumb|900px|Fig.4 Simplified scheme of the central circadian oscillator system.[2]]] </th><th></table><br> |
+ | ===Reference=== | ||
+ | [1]Akiyama S. Structural and dynamic aspects of protein clocks: how can they be so slow and stable?[J]. Cellular & Molecular Life Sciences Cmls, 2012, 69(13):2147-2160.<br> | ||
+ | [2]Paddock, Mark, Boyd, et al. Active Output State of the Synechococcus Kai Circadian Oscillator[J]. Pnas, 2014, 110(40):E3849-E3857. | ||
− | + | More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results <br> | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 19:53, 17 October 2018
KaiC coding region
Summary
It is the core part of the Kai circuit, acting as a hexamer. This part can be both auto phosphorylated and dephosphorylated, and it can achieve about 24 hours of phosphorylation-dephosphorylation oscillation under the regulation of KaiA and KaiB.
Each KaiC monomer has two subunits, N-terminal CI subunit and C-terminal CII subunit. Each CII subunit has 431 serine (S431) residue and 432 threonine (T432) residue phosphorylation sites(Fig3). Therefore, KaiC monomer has four possible phosphorylation states, showing different functions. SasA proteins will affect downstream signaling pathways, when it is phosphorylated by KaiC hexamers in a specific phosphorylated state.
A separate fragment is 1560bp.
Structure of KaiC
KaiC's phosphorylation stage
Reference
[1]Akiyama S. Structural and dynamic aspects of protein clocks: how can they be so slow and stable?[J]. Cellular & Molecular Life Sciences Cmls, 2012, 69(13):2147-2160.
[2]Paddock, Mark, Boyd, et al. Active Output State of the Synechococcus Kai Circadian Oscillator[J]. Pnas, 2014, 110(40):E3849-E3857.
More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 148
Illegal AgeI site found at 859
Illegal AgeI site found at 868
Illegal AgeI site found at 874
Illegal AgeI site found at 1198
Illegal AgeI site found at 1225 - 1000COMPATIBLE WITH RFC[1000]