Difference between revisions of "Part:BBa K2570000"

Latest revision as of 04:32, 10 October 2018

PAR

To optimize the engineered 2-PE biosynthetic pathway, the Par (BAG13450.2) the phenyla- cetaldehyde reductase from the rose (Rosa sp.)

Construction and optimization of the 2-PE biosynthetic pathway in E.coli.BBa_K2570010

A heterologous Ehrlich pathway was constructed for 2-PE bio-synthesis in E. coli by co-overexpressing the aromatic transaminase from E. coli (TyrB, WP032305522.1)BBa_K2570002, phenylpyruvate decarboxylase from S. cerevisiae (Aro10, NP 010668.3)BBa_K2570001 and phenyla- cetaldehyde reductase from the rose (Par , BAG13450.2 ) under the control of the constitutive promoter BBa_J23100. The resulting E.coli strain BW25113 pSB1C3-Aro10-PAR-TyrB was used as the whole-cell biocatalyst, and 2-PE was obtained from 50mM L-Phe in a 12 h bioconversion .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 247
Illegal NotI site found at 610
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 To optimize the engineered 2-PE biosynthetic pathway,  the Par (BAG13450.2) the phenyla- cetaldehyde reductase from the rose (Rosa sp.)
-Construction and optimization of the 2-PE biosynthetic pathway in E.coli.
+Construction and optimization of the 2-PE biosynthetic pathway in  ''E.coli''.[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570010 BBa_K2570010]
-A heterologous Ehrlich pathway was constructed for 2-PE bio-synthesis in E. coli by co-overexpressing the aromatic transaminase from E. coli (TyrB, WP032305522.1), phenylpyruvate decarboxylase from S. cerevisiae (Aro10, NP 010668.3) and phenyla- cetaldehyde reductase from the rose (Par , BAG13450.2 ) under the control of the constitutive promoter (BBa_J23100). The resulting E.coli strain BW25113 pSB1C3-Aro10-PAR-TyrB was used as the whole-cell biocatalyst, and 2-PE was obtained from 50mM L-Phe in a 12 h bioconversion .
+A heterologous Ehrlich pathway was constructed for 2-PE bio-synthesis in ''E. coli'' by co-overexpressing the aromatic transaminase from ''E. coli'' (TyrB, WP032305522.1)[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570002 BBa_K2570002], phenylpyruvate decarboxylase from ''S. cerevisiae'' (Aro10, NP 010668.3)[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570001 BBa_K2570001] and phenyla- cetaldehyde reductase from the rose (Par , BAG13450.2 ) under the control of the constitutive promoter [https://parts.igem.org/wiki/index.php?title=Part:BBa_J23100 BBa_J23100]. The resulting ''E.coli'' strain BW25113 pSB1C3-Aro10-PAR-TyrB was used as the whole-cell biocatalyst, and 2-PE was obtained from 50mM L-Phe in a 12 h bioconversion .