Difference between revisions of "Part:BBa K2570007:Design"

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===Design Notes===
 
===Design Notes===
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The atoA sequence obtained from E.coli Bw25113 by PCR
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 +
Primers:
 +
 
 +
Fwd: 5’-gcaaataactcgagaaggagatataatggatgcgaaacaacgtattg-3’
 +
 
 +
Rev: 5’-ttggtaactttcattatatctcctttcataaatcaccccgttgcg-3’
  
  
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===Source===
 
===Source===
  
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Organism="Escherichia coli str. K-12 substr. MG1655". EC_number="2.8.3.9". Protein_id="AAC75281.1".  
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Organism="''Escherichia coli str. K-12 substr. MG1655''". EC_number="2.8.3.9". Protein_id="AAC75281.1".  
 
https://www.ncbi.nlm.nih.gov/nuccore/U00096.3?from=2324109&to=2324759
 
https://www.ncbi.nlm.nih.gov/nuccore/U00096.3?from=2324109&to=2324759
  
 
===References===
 
===References===
 +
Saini M, Chen M H, Chiang C J, et al. Potential production platform of n-butanol in Escherichia coli[J]. Metabolic Engineering, 2015, 27:76.

Latest revision as of 08:46, 16 October 2018


atoA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 180
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers:

Fwd: 5’-gcaaataactcgagaaggagatataatggatgcgaaacaacgtattg-3’

Rev: 5’-ttggtaactttcattatatctcctttcataaatcaccccgttgcg-3’


Source

Organism="Escherichia coli str. K-12 substr. MG1655". EC_number="2.8.3.9". Protein_id="AAC75281.1". https://www.ncbi.nlm.nih.gov/nuccore/U00096.3?from=2324109&to=2324759

References

Saini M, Chen M H, Chiang C J, et al. Potential production platform of n-butanol in Escherichia coli[J]. Metabolic Engineering, 2015, 27:76.