Difference between revisions of "Part:BBa K2856001:Design"

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===Design Notes===
 
===Design Notes===
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there are  EcoRI，XbaI,SpeI restriction sites in original coding squence.So we ask the company to de novo synthsis this squence to remove
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Sequence has been optimized for expression in Lactococcus lactis. We remove some illegal restriction sites in original squence and use ATG as
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the illegal resitriction sites
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initiation codon.
  
 
===Source===
 
===Source===
  
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this gene is amplified from Streptococcus thermophilus genome
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The original complete CDS sequence can be obtained from Genebank. Genebank accession: AP018935.1
  
 
===References===
 
===References===

Latest revision as of 02:39, 1 October 2018


Bifunctional glutamate-cysteine ligase/glutathione synthase (gshF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence has been optimized for expression in Lactococcus lactis. We remove some illegal restriction sites in original squence and use ATG as initiation codon.

Source

The original complete CDS sequence can be obtained from Genebank. Genebank accession: AP018935.1

References