Difference between revisions of "Part:BBa K2623002"
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<partinfo>BBa_K2623002 short</partinfo>
 
<partinfo>BBa_K2623002 short</partinfo>
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===Summary===
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It is a component of the Kai circuit, which inhibits the activation of KaiA to active KaiC ’s autophosphorylation, thereby enabling KaiC to dephosphorylate itself.
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It is a component of the Kai circuit, which inhibits the activation of KaiA to active KaiC ’s autophosphorylation, thereby enabling KaiC to dephosphorylate itself.<br>
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Component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. The KaiABC complex may act as a promoter-non-specific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. In the complex, it decreases the phosphorylation status of KaiC. It has no effect on KaiC by itself, but instead need the presence of both KaiA and KaiC, suggesting that it acts by antagonizing the interaction between KaiA and KaiC.<br>
 
A separate fragment is 309bp.
 
A separate fragment is 309bp.
  
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===Structure of KaiB===
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<table><tr><th>
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[[Image:KaiB.png|thumb|400px|Fig.1 Protein structure of KaiB]]</th><th></table>
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===KaiB's function in KaiC's phosphorylation stage===
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<table><tr><th>
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[[Image:Kai_1_.gif|thumb|900px|Fig.2 Simplified scheme of the central circadian oscillator system.[1]]]</th><th></table>
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===Reference===
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[1]Paddock, Mark, Boyd, et al. Active Output State of the Synechococcus Kai Circadian Oscillator[J]. Pnas, 2014, 110(40):E3849-E3857.
  
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More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results <br>
 
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 19:51, 17 October 2018


KaiB coding region

Summary

It is a component of the Kai circuit, which inhibits the activation of KaiA to active KaiC ’s autophosphorylation, thereby enabling KaiC to dephosphorylate itself.
Component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. The KaiABC complex may act as a promoter-non-specific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. In the complex, it decreases the phosphorylation status of KaiC. It has no effect on KaiC by itself, but instead need the presence of both KaiA and KaiC, suggesting that it acts by antagonizing the interaction between KaiA and KaiC.
A separate fragment is 309bp.

Structure of KaiB

Fig.1 Protein structure of KaiB

KaiB's function in KaiC's phosphorylation stage

Fig.2 Simplified scheme of the central circadian oscillator system.[1]

Reference

[1]Paddock, Mark, Boyd, et al. Active Output State of the Synechococcus Kai Circadian Oscillator[J]. Pnas, 2014, 110(40):E3849-E3857.

More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]