Difference between revisions of "Part:BBa K2762004:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The whole genome sequence of Synechococcus sp. PCC7002 is found in NCBI. We first codon optimized the CA sequence and sent it to IDT for gene synthesis. | + | The whole genome sequence of <i>Synechococcus sp.</i> PCC7002 is found in NCBI. We first codon optimized the CA sequence and sent it to IDT for gene synthesis. |
The gene fragment is amplified via PCR reaction for cloning. | The gene fragment is amplified via PCR reaction for cloning. | ||
===Source=== | ===Source=== | ||
+ | <i>Synechococcus elongatus</i> PCC7002 | ||
https://www.ncbi.nlm.nih.gov/nuccore/169884305 | https://www.ncbi.nlm.nih.gov/nuccore/169884305 | ||
===References=== | ===References=== |
Latest revision as of 10:14, 16 October 2018
CcaA (formerly icfA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The whole genome sequence of Synechococcus sp. PCC7002 is found in NCBI. We first codon optimized the CA sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning.
Source
Synechococcus elongatus PCC7002
https://www.ncbi.nlm.nih.gov/nuccore/169884305