Difference between revisions of "Part:BBa K2656019"

Latest revision as of 01:35, 18 October 2018

LuxI Coding Sequence

Figure 1. DNA basic parts domestication. Third construction corresponds with CDS Basic Part adaptation into the GoldenBraid grammar.

Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence BBa_C0161 adapted to the Golden Braid grammar and so used with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design Golden Gate] assembly methods. It was codon optimized for E. coli.

LuxI coding sequence codifies for the 3OC6HSL (AHL) synthetase, so two signaling molecules of AHL interacts with two luxR proteins creating a complex which binds to the pLux promoter.

This part can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a one-step reaction with the Golden Gate assembly protocol .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K2656019 short</partinfo>
+[[File:T--Valencia_UPV--im11UPV2018.png|200px|thumb|right|alt=domestication.|Figure 1. DNA basic parts domestication. Third construction corresponds with CDS Basic Part adaptation into the GoldenBraid grammar. ]]
+Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence [https://parts.igem.org/Part:BBa_C0161  BBa_C0161] adapted to the Golden Braid grammar and so used with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design Golden Gate] assembly methods. It was codon optimized for ''E. coli''.
+LuxI coding sequence codifies for the 3OC6HSL (AHL) synthetase, so two signaling molecules of AHL interacts with two luxR proteins creating a complex which binds to the pLux promoter.
-Part BBa_C0061 standarized as Phytobrick. Acyl-homoserine-lactone synthase. It can be used to assemble transcriptional units with the Golden Gate assembly method, and also, it can be used as a Biobrick.
+This part can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a one-step reaction with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>.
 <!-- Add more about the biology of this part here