Difference between revisions of "Part:BBa K2570002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | <span style="font-weight:bold;">Site-directed mutation site </span> | |
| + | TyrB（Pst1：CTGCAG—CTGCAA） | ||
| + | sense primer: GGTGGCTGCAATGCTGAATGACG | ||
| + | |||
| + | Anti-sense primer: TCAGCATTGCAGCCACCACCTG | ||
===Source=== | ===Source=== | ||
Phenylalanine aminotransferase Tyrb from Escherichia coli 21B. | Phenylalanine aminotransferase Tyrb from Escherichia coli 21B. | ||
| + | |||
| + | https://www.ncbi.nlm.nih.gov/protein/WP_032305522.1?report=genbank&log$=protalign&blast_rank=3&RID=V13P17MW014 | ||
===References=== | ===References=== | ||
Latest revision as of 09:34, 5 October 2018
TyrB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1054
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Site-directed mutation site
TyrB（Pst1：CTGCAG—CTGCAA）
sense primer: GGTGGCTGCAATGCTGAATGACG
Anti-sense primer: TCAGCATTGCAGCCACCACCTG
Source
Phenylalanine aminotransferase Tyrb from Escherichia coli 21B.