Difference between revisions of "Part:BBa K2598005:Design"

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===Design Notes===
 
===Design Notes===
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We use to regulate gene expression via green light
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The sequence is optimized to delete illegal restriction sites and render it PFC compatible.
  
  
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Tabor, J. J., Levskaya, A. & Voigt, C. A. Multichromatic control of gene expression in Escherichia coli. J
 
Tabor, J. J., Levskaya, A. & Voigt, C. A. Multichromatic control of gene expression in Escherichia coli. J
 
Mol Biol 405, 315-324, (2011).
 
Mol Biol 405, 315-324, (2011).
 +
 +
Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)

Latest revision as of 10:06, 13 October 2018


CcaS_CcaR Green light sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 676
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2774
    Illegal XhoI site found at 2322
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1340
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is optimized to delete illegal restriction sites and render it PFC compatible.


Source

It's from the genome of Synechocystis sp. PCC6803.

References

Tabor, J. J., Levskaya, A. & Voigt, C. A. Multichromatic control of gene expression in Escherichia coli. J Mol Biol 405, 315-324, (2011).

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)