Difference between revisions of "Part:BBa K2560007:Design"

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===Design Notes===
 
===Design Notes===
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The sequence was obtained from part BBa_J23100. The parts sequence was not changed apart from adding the promotor overhangs that are required for subsequent cloning.
 
  
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<img style="width:50%" src="https://parts.igem.org/File:Promoter_characterization_Marburg_Toolbox.pdf#file">
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<html>
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The sequence was obtained from part <a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>. The parts sequence was not changed apart from adding the promoter overhangs that are required for subsequent cloning.
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</html>
  
 
===Source===
 
===Source===
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The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector (BBa_K2560002) using Golden Gate assembly.
 
  
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Forward Oligo:
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<html>
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The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. If you stuggle with <i> de novo </i> synthesis we recomended this
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<a href="https://parts.igem.org/Help:Promoters/Construction">site</a>.
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</html>
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<b> Forward Oligo:</b>
 
CTCGGGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTACT
 
CTCGGGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTACT
  
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Reverse Oligo:
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<b> Reverse Oligo:</b>
 
CTCAAGTAGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCC
 
CTCAAGTAGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCC
  
 
===References===
 
===References===

Latest revision as of 19:50, 12 October 2018


Phytobrick version of BBa_J23100


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was obtained from part BBa_J23100. The parts sequence was not changed apart from adding the promoter overhangs that are required for subsequent cloning.

Source

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward Oligo: CTCGGGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTACT

Reverse Oligo: CTCAAGTAGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCC

References