Difference between revisions of "Part:BBa K2656017"

 
 
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<partinfo>BBa_K2656017 short</partinfo>
 
<partinfo>BBa_K2656017 short</partinfo>
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[[File:T--Valencia_UPV--im11UPV2018.png|200px|thumb|right|alt=domestication.|Figure 1. DNA basic parts domestication. Third construction corresponds with CDS Basic Part adaptation into the GoldenBraid grammar. ]]
  
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Part BBa_K2442103 domesticated to the Golden Braid 3.0 grammar. It works as a transcription factor for pBad promoters when it is activated by L-Arabinose. It can be used to assembly transcriptional units with the Golden Gate assembly method, and also, it can be used as a Biobrick.
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BBa_K2656017 is the transcription factor AraC coding sequence [https://parts.igem.org/Part:BBa_K2442103 BBa_K2442103] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods.  
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It acts negatively on the pBad promoter when it is not activated by L-Arabinose.
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This piece can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>.
  
 
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Latest revision as of 02:30, 18 October 2018


AraC TF Coding Sequence

domestication.
Figure 1. DNA basic parts domestication. Third construction corresponds with CDS Basic Part adaptation into the GoldenBraid grammar.

BBa_K2656017 is the transcription factor AraC coding sequence BBa_K2442103 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. It acts negatively on the pBad promoter when it is not activated by L-Arabinose.

This piece can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the Golden Gate assembly protocol .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]