Difference between revisions of "Part:BBa K2609003:Design"

 
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===Design Notes===
 
===Design Notes===
−
The sequence was codon optimized for expression in E. coli. The following changes were made to the original sequence. ____
+
The sequence was codon optimized for expression in <i>E. coli</i>.
−
 
+
The following changes were made to the wild-type protein sequence.  
 +
____
  
 +
A double stop codon is placed at the end to ensure proper termination.
  
 
===Source===
 
===Source===
  
−
T4 bacteriophage genome, sequence from GenBank (https://www.ncbi.nlm.nih.gov/nuccore/HM137666)
+
T4 bacteriophage genome, sequence from GenBank: https://www.ncbi.nlm.nih.gov/nuccore/HM137666
  
 
===References===
 
===References===
 +
[1] Leiman, Petr G., et al. "Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host." Cell 118.4 (2004): 419-429.<br>
 +
[2] Yoichi, Masatoshi, et al. "Alteration of tail fiber protein gp38 enables T2 phage to infect Escherichia coli O157: H7." Journal of biotechnology 115.1 (2005): 101-107.<br>
 +
[3] Rakhuba, D. V., et al. "Bacteriophage receptors, mechanisms of phage adsorption and penetration into host cell." Pol. J. Microbiol 59.3 (2010): 145-155.<br>
 +
[4] Bartual, Sergio G., et al. "Structure of the bacteriophage T4 long tail fiber receptor-binding tip." Proceedings of the National Academy of Sciences 107.47 (2010): 20287-20292.

Latest revision as of 05:33, 2 October 2018


T4 bacteriophage long tail fiber protein gp37 (Modification II)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 424
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for expression in E. coli. The following changes were made to the wild-type protein sequence. ____

A double stop codon is placed at the end to ensure proper termination.

Source

T4 bacteriophage genome, sequence from GenBank: https://www.ncbi.nlm.nih.gov/nuccore/HM137666

References

[1] Leiman, Petr G., et al. "Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host." Cell 118.4 (2004): 419-429.
[2] Yoichi, Masatoshi, et al. "Alteration of tail fiber protein gp38 enables T2 phage to infect Escherichia coli O157: H7." Journal of biotechnology 115.1 (2005): 101-107.
[3] Rakhuba, D. V., et al. "Bacteriophage receptors, mechanisms of phage adsorption and penetration into host cell." Pol. J. Microbiol 59.3 (2010): 145-155.
[4] Bartual, Sergio G., et al. "Structure of the bacteriophage T4 long tail fiber receptor-binding tip." Proceedings of the National Academy of Sciences 107.47 (2010): 20287-20292.