Difference between revisions of "Part:BBa K2865000:Design"

 
 
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===Design Notes===
 
===Design Notes===
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We selected this range of sequence for it contains several regulatory elements and could be activated by a few factors, including endothelin (ET), angiotensin II (Ang II), and hypoxia.
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In vivo studies of BNP stress-responsive regions revealed that -408/+100 bp hBNP promoter fragment is able to increase luciferase reporter activity after acute MI and AngⅡ infusion.
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===Source===
 
===Source===
  
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This promoter is synthesized from Homo sapiens natriuretic peptide precursor B (NPPB) gene.
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approximal region (-408/+100bp) of BNP promoter
  
 
===References===
 
===References===

Latest revision as of 15:12, 8 October 2018


BNP promoter, heart failure inducible


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 242


Design Notes

In vivo studies of BNP stress-responsive regions revealed that -408/+100 bp hBNP promoter fragment is able to increase luciferase reporter activity after acute MI and AngⅡ infusion.


Source

approximal region (-408/+100bp) of BNP promoter

References