Difference between revisions of "Part:BBa K2587000"

Latest revision as of 23:01, 17 October 2018

luxI_codon optimized S.cerevisiae

LuxI is an acyl homoserine lactone synthase, most known from the bacterium Aliivibrio fischeri. LuxI is a basic component of the quorum sensing system of A. fischeri. Bacteria use it as a communication module to regulate expression of genes in a cell density-dependent manner. This part contains a variant of this synthase codon optimized for the common yeast, Saccharomyces cerevisiae. Besides the codon optimization, the construct also contains type II S restriction sites (BsaI), useful for example for the Golden Gate assembly cloning method. Since yeast is a eukaryotic organism and the quorum sensing system is encountered in prokaryotes, a codon optimized variant for utilization in an eukaryotic organism represents the beginning of designing quorum sensing-based control systems in eukaryotes. For example, LuxI is coupled with the design of a synthetic promoter, which is supposed to be activated by binding of the quorum sensing molecule together with the regulator (LuxR) to the promoter to induce expression of a reporter gene or a lysis gene to control cell population.

Usage and Biology

This part can be used as a component for designing synthetic circuits in S.cerevisiae. For instance, in our project, we used this gene to induce expression of a reporter after activation of a synthetic, self designed promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 6
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 689
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 30
Illegal BsaI.rc site found at 673

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2587000 short</partinfo>
-LuxI is an acyl homoserine lactone synthase, most known from the bacterium Alivibrio fischeri. LuxI is a basic component of the quorum sensing system of V.fischeri. In bacteria it is used as a communication module among those organisms to regulate expression. In this case the synthase is codon optimised for the common yeast, Saccharomyces cerevisiae. Moreover besides the codon optimisation the construct contains also type II S cutting sites (BsaI), useful for example for Golden Gate cloning method. In yeast quorum sensing system is not frequently encountered and therefore a codon optimised form for utilisation in an eukaryotic organism represents a beginning to design control systems in this type of organisms. For example, this LuxI is coupled in our case with the design of a synthetic promoter, which is supposed to be activated by binding of the quorum sensing molecule together with the regulator (LuxR) to the promoter to induce expression of a reporter or a lysis gene to control cell population.
+LuxI is an acyl homoserine lactone synthase, most known from the bacterium <i>Aliivibrio fischeri</i>. LuxI is a basic component of the quorum sensing system of <i>A. fischeri</I>. Bacteria use it as a communication module  to regulate expression of genes in a cell density-dependent manner. This part contains a variant of this synthase codon optimized for the common yeast, <i>Saccharomyces cerevisiae</I>. Besides the codon optimization, the construct also contains type II S restriction sites (<i>Bsa</i>I), useful for example for the Golden Gate assembly cloning method. Since yeast is a eukaryotic organism and the quorum sensing system is encountered in prokaryotes, a codon optimized variant for utilization in an eukaryotic organism represents the beginning of designing quorum sensing-based control systems in eukaryotes. For example, LuxI is coupled with the design of a synthetic promoter, which is supposed to be activated by binding of the quorum sensing molecule together with the regulator (LuxR) to the promoter to induce expression of a reporter gene or a lysis gene to control cell population.
-<!-- Add more about the biology of this part here
+<b>Usage and Biology</b>
-===Usage and Biology===
+This part can be used as a component for designing synthetic circuits in <i>S.cerevisiae</i>. For instance, in our project, we used this gene to induce expression of a reporter after activation of a synthetic, self designed promoter.
 <!-- -->