Difference between revisions of "Part:BBa K2729000"
 
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===Usage and Biology===
 
===Usage and Biology===
 
Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.  
 
Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.  
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<span class='h3bb'>Sequence and Features</span>
 
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Mature DENV capsid is a highly basic protein of 12 kDa that forms homodimers in solution with affinity for both nucleic acids and lipid membranes. The 100-residue monomer contains 26 basic amino acids and only 3 acidic residues.
  
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===Characterization===
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===Functional Parameters===
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===Reference===
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The sequence is derived from native dengue virus and optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
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- [https://www.ncbi.nlm.nih.gov/gene/507572 NCBI Gene Database]
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[[File:Tokyotech electrophoresis.png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis (Marker: lambda HindIII)''' ]]
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[[File:T--Tokyo_Tech--test-electrop.gif|thumb|left|600px| '''Figure 1:''' '''Result of the qualitative experiment''' - Cells were grown at room temperature on LB agar plates with and without iP. β-galactosidase activity was monitored by X-gal. Photographs were taken after 25h incubation. ]]
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As shown in Fig. 1, blue color was developed only when cells carried the AHK4 expressing plasmid and when the medium contained 100 µM iP. Therefore, we concluded that AHK4 could receive iP and downstream AHK4→RcsD→RscB→cps::lacZ pathway was activated as expected.  
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As shown in Figure 1, the band of DENV1 C come around 300 bp. It corresponds to its actual length.
 
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===Analysis===
  
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Our team successfully validated that the sequence of DENV1 C was synthesized, and obtained enough amount of it for the transfection.
  
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If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.
  
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===Sequence and Features===
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<partinfo>BBa_K2729000 SequenceAndFeatures</partinfo>
  
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===Reference===
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- [https://www.ncbi.nlm.nih.gov/gene/507572 NCBI Gene Database]
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- [https://www.ncbi.nlm.nih.gov/pubmed/?term=Properties+and+Functions+of+the+Dengue+Virus+Capsid+Protein Properties and Functions of the Dengue Virus Capsid Protein.]

Latest revision as of 03:32, 18 October 2018


DENV1 C (Capsid Protein | Dengue Virus Serotype I)

Usage and Biology

Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Mature DENV capsid is a highly basic protein of 12 kDa that forms homodimers in solution with affinity for both nucleic acids and lipid membranes. The 100-residue monomer contains 26 basic amino acids and only 3 acidic residues.

Characterization

The sequence is derived from native dengue virus and optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the Electrophoresis (Marker: lambda HindIII)


As shown in Figure 1, the band of DENV1 C come around 300 bp. It corresponds to its actual length.

Analysis

Our team successfully validated that the sequence of DENV1 C was synthesized, and obtained enough amount of it for the transfection.

If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

- NCBI Gene Database

- Properties and Functions of the Dengue Virus Capsid Protein.