Difference between revisions of "Part:BBa K2729000"
 
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<partinfo>BBa_K2729000 short</partinfo>
 
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Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
 
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===Usage and Biology===
 
===Usage and Biology===
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Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
  
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Mature DENV capsid is a highly basic protein of 12 kDa that forms homodimers in solution with affinity for both nucleic acids and lipid membranes. The 100-residue monomer contains 26 basic amino acids and only 3 acidic residues.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2729000 SequenceAndFeatures</partinfo>
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===Characterization===
  
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The sequence is derived from native dengue virus and optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
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===Functional Parameters===
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<partinfo>BBa_K2729000 parameters</partinfo>
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[[File:Tokyotech electrophoresis.png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis (Marker: lambda HindIII)''' ]]
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アップロードした図・表ファイルコード<br>
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図・表の右側に表示したい文字列
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<br style="clear: both" />
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例
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[[File:Tokyo_Tech_AHK4ql.png|thumb|left|600px| '''Figure 1:''' '''Result of the qualitative experiment''' - Cells were grown at room temperature on LB agar plates with and without iP. β-galactosidase activity was monitored by X-gal. Photographs were taken after 25h incubation. ]]
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As shown in Fig. 1, blue color was developed only when cells carried the AHK4 expressing plasmid and when the medium contained 100 µM iP. Therefore, we concluded that AHK4 could receive iP and downstream AHK4→RcsD→RscB→cps::lacZ pathway was activated as expected.  
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As shown in Figure 1, the band of DENV1 C come around 300 bp. It corresponds to its actual length.
 
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===Analysis===
  
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Our team successfully validated that the sequence of DENV1 C was synthesized, and obtained enough amount of it for the transfection.
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文頭に入力すると文頭にXピクセルのマージンが入る（Xには好みの数字を入力）
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<i>斜体にしたい文字列</i>
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If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.
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斜体にしたい文字列を<i>　</i>で挟むことで斜体にする（学名、遺伝子名等）
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’’’太字にしたい文字’’’
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===Sequence and Features===
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太字にしたい文字列をダッシュ（’’’）で挟むことで太字にする
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<partinfo>BBa_K2729000 SequenceAndFeatures</partinfo>
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文頭に入力することで箇条書きのコロンになる
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===Reference===
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- [https://www.ncbi.nlm.nih.gov/gene/507572 NCBI Gene Database]
  
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see the[リンク先URLをここに入力“本文中でのリンクを埋め込みたい文字"] page
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- [https://www.ncbi.nlm.nih.gov/pubmed/?term=Properties+and+Functions+of+the+Dengue+Virus+Capsid+Protein Properties and Functions of the Dengue Virus Capsid Protein.]
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例
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see the [http://2017.igem.org/Team:TokyoTech/Experiment/Chimeric_Transcription_Factor “Chemeric Transcription Factor”]page
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と入力すると
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こんな感じになる
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see the “Chemeric Transcription Factor”page
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Latest revision as of 03:32, 18 October 2018


DENV1 C (Capsid Protein | Dengue Virus Serotype I)

Usage and Biology

Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Mature DENV capsid is a highly basic protein of 12 kDa that forms homodimers in solution with affinity for both nucleic acids and lipid membranes. The 100-residue monomer contains 26 basic amino acids and only 3 acidic residues.

Characterization

The sequence is derived from native dengue virus and optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the Electrophoresis (Marker: lambda HindIII)


As shown in Figure 1, the band of DENV1 C come around 300 bp. It corresponds to its actual length.

Analysis

Our team successfully validated that the sequence of DENV1 C was synthesized, and obtained enough amount of it for the transfection.

If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

- NCBI Gene Database

- Properties and Functions of the Dengue Virus Capsid Protein.