Difference between revisions of "Part:BBa K2729000"

Latest revision as of 03:32, 18 October 2018

DENV1 C (Capsid Protein | Dengue Virus Serotype I)

Usage and Biology

Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Mature DENV capsid is a highly basic protein of 12 kDa that forms homodimers in solution with affinity for both nucleic acids and lipid membranes. The 100-residue monomer contains 26 basic amino acids and only 3 acidic residues.

Characterization

The sequence is derived from native dengue virus and optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the Electrophoresis (Marker: lambda HindIII)

As shown in Figure 1, the band of DENV1 C come around 300 bp. It corresponds to its actual length.

Analysis

Our team successfully validated that the sequence of DENV1 C was synthesized, and obtained enough amount of it for the transfection.

If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Reference

- NCBI Gene Database

- Properties and Functions of the Dengue Virus Capsid Protein.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2729000 short</partinfo>
-Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
-<!-- -->
+Mature DENV capsid is a highly basic protein of 12 kDa that forms homodimers in solution with affinity for both nucleic acids and lipid membranes. The 100-residue monomer contains 26 basic amino acids and only 3 acidic residues.
-<span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K2729000 SequenceAndFeatures</partinfo>
+===Characterization===
-<!-- Uncomment this to enable Functional Parameter display
+The sequence is derived from native dengue virus and optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
-===Functional Parameters===
-<partinfo>BBa_K2729000 parameters</partinfo>
-<!-- -->
-<span style="margin-left: Xpx;">
+[[File:Tokyotech electrophoresis.png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis (Marker: lambda HindIII)'''  ]]
-文頭に入力すると文頭にXピクセルのマージンが入る（Xには好みの数字を入力）
-<i>斜体にしたい文字列</i>
+<br>
-斜体にしたい文字列を<i>　</i>で挟むことで斜体にする（学名、遺伝子名等）
+As shown in Figure 1, the band of DENV1 C come around 300 bp. It corresponds to its actual length.
+<br style="clear: both" />
-’’’太字にしたい文字’’’
+===Analysis===
-太字にしたい文字列をダッシュ（’’’）で挟むことで太字にする
-＊
+Our team successfully validated that the sequence of DENV1 C was synthesized, and obtained enough amount of it for the transfection.
-文頭に入力することで箇条書きのコロンになる
+If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.
+===Sequence and Features===
+<partinfo>BBa_K2729000 SequenceAndFeatures</partinfo>
+===Reference===
+- [https://www.ncbi.nlm.nih.gov/gene/507572 NCBI Gene Database]
-see the[リンク先URLをここに入力“本文中でのリンクを埋め込みたい文字"] page
+- [https://www.ncbi.nlm.nih.gov/pubmed/?term=Properties+and+Functions+of+the+Dengue+Virus+Capsid+Protein Properties and Functions of the Dengue Virus Capsid Protein.]
-例
- see the [http://2017.igem.org/Team:TokyoTech/Experiment/Chimeric_Transcription_Factor “Chemeric Transcription Factor”]page
-と入力すると
-こんな感じになる
- see the “Chemeric Transcription Factor”page