Difference between revisions of "Part:BBa K2560034:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | <html> | ||
+ | This part was created by Overlap extension polymerase chain reaction and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. | ||
+ | </html> | ||
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+ | <b> Forward oligo:</b> | ||
+ | AACGTCTCGCTCGGCTTAACCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCC | ||
+ | |||
+ | <b> Reverse Oligo:</b> | ||
+ | TTCGTCTCCCTCAAGCGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTG | ||
===Source=== | ===Source=== | ||
+ | <html> | ||
− | + | As source we used the part <a href="https://parts.igem.org/Part:BBa_B0010">BBa_B0010</a>. | |
− | + | </html> |
Latest revision as of 01:44, 18 October 2018
Phytobrick version of BBa_B0010
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was created by Overlap extension polymerase chain reaction and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly.
Forward oligo: AACGTCTCGCTCGGCTTAACCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCC
Reverse Oligo: TTCGTCTCCCTCAAGCGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTG
Source
As source we used the part BBa_B0010.