Difference between revisions of "Part:BBa K2586019:Design"

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===Design Notes===
 
===Design Notes===
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We inserted the ordered GAT-enzyme into the <i>PstI</i> and <i>EcoRI</i> digested <b>pSB1C3</b> backbone
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The sequence of the GAT-enzyme was ordered from Integrated DNA Technologies [https://eu.idtdna.com/pages (IDT)]. We used self designed primers to create the fitting ends and inserted the so prepared part as template into the <i>PstI</i> and <i>EcoRI</i> digested <b>pSB1C3</b> backbone.
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===Source===
 
===Source===
  
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This part was first described in: Castle <i>et. al.</i> paper (2004). We ordered the gene from IDT (Integrated DNA Technologies) and used it as insert for our experiments.  
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This part was first described in: Castle <i>et. al.</i> paper (2004). We took this paper as base for the experimental process under the hypothesis that GAT could potentially be inserted into <i>Bacillus subtilis</i> to enable the bacteria to inactivate glyphosate.
  
 
===References===
 
===References===
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<a href="http://science.sciencemag.org/content/304/5674/1151">Castle <i> et. al. </i> 2004</a>
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[https://www.ncbi.nlm.nih.gov/pubmed/15155947 (Castle <i>et. al.</i> 2004)]: Discovery and Directed Evolution of a Glyphosate Tolerance Gene
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(1) Castle <i>et. al.</i> 2004: Discovery and Directed Evolution of a Glyphosate Tolerance Gene
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Latest revision as of 11:00, 13 September 2018


GAT: Glyphosate N-Acetyltransferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of the GAT-enzyme was ordered from Integrated DNA Technologies (IDT). We used self designed primers to create the fitting ends and inserted the so prepared part as template into the PstI and EcoRI digested pSB1C3 backbone.

Source

This part was first described in: Castle et. al. paper (2004). We took this paper as base for the experimental process under the hypothesis that GAT could potentially be inserted into Bacillus subtilis to enable the bacteria to inactivate glyphosate.

References

(Castle et. al. 2004): Discovery and Directed Evolution of a Glyphosate Tolerance Gene