Difference between revisions of "Part:BBa K2586000"

Latest revision as of 11:08, 11 September 2018

Palf4: Constitutively active promoter for Bacillus subtilis

Palf4 is an artificial promoter that is constitutively active in Bacillus subtilis. The part has the advantage that it consists of a short DNA sequence (30 bp). The promoter sequence can be fused to reporter genes, such as lacZ as well as to any other gene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2586000 short</partinfo>
-<i>Palf4</i> is an artificial promoter that is constitutively expressed in <i>Bacillus subtilis</i>. It has the advantage to have only a small DNA sequence of 30 bp length.
+<i>Palf4</i> is an artificial promoter that is constitutively active in <i>Bacillus subtilis</i>. The part has the advantage that it consists of a short DNA sequence (30 bp).
-The promoter sequence can be fused with <i>lacz</i> and a riboswitch, to carry out experiments with the characteristic enzyme &#946;-Galactosidase.
+The promoter sequence can be fused to reporter genes, such as <i>lacZ</i> as well as to any other gene.
-Artificial promoter, which is consttuitevely active in B. subtilis. Its advantage lays in the short sequence, which allows it to be introduced into genetic systems with ease.
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