Difference between revisions of "Part:BBa K2586000"
 
(2 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2586000 short</partinfo>
 
<partinfo>BBa_K2586000 short</partinfo>
  
−
<i>Palf4</i> is an artificial promoter that is constitutively expressed in <i>Bacillus subtilis</i>. It has the advantage to have only a small DNA sequence of 30 bp length.  
+
<i>Palf4</i> is an artificial promoter that is constitutively active in <i>Bacillus subtilis</i>. The part has the advantage that it consists of a short DNA sequence (30 bp).  
−
The promoter sequence can be fused with <i>lacz</i> and a riboswitch, to carry out experiments with the characteristic enzyme &#946;-Galactosidase.  
+
The promoter sequence can be fused to reporter genes, such as <i>lacZ</i> as well as to any other gene.  
  
−
Artificial promoter, which is consttuitevely active in B. subtilis. Its advantage lays in the short sequence, which allows it to be introduced into genetic systems with ease.
 
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
−
===We used the part to check the expression of other parts, like <a ref="https://parts.igem.org/Part:BBa_K2586001">BBa_K2586001</a>===
+
======
  
 
<!-- -->
 
<!-- -->

Latest revision as of 11:08, 11 September 2018


Palf4: Constitutively active promoter for Bacillus subtilis

Palf4 is an artificial promoter that is constitutively active in Bacillus subtilis. The part has the advantage that it consists of a short DNA sequence (30 bp). The promoter sequence can be fused to reporter genes, such as lacZ as well as to any other gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]