Difference between revisions of "Part:BBa K2586001:Design"

Latest revision as of 11:04, 13 September 2018

GltT: glutamate and glyphosate transporter in B. subtilis

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 742
Illegal NgoMIV site found at 1123
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone.

Source

This basic part consists a gene that was amplified by PCR using chromosomal DNA of the wild type strain B. subtilis 168 (Laboratory collection AG Commichau, Department for General Microbiology, University Göttingen, Germany).

References

(Zeigler et al. 2008): The origins of 168, W23, and other Bacillus subtilis legacy strains. J. Bacteriol. 190: 6983-95.

@@ Line 7: / Line 7: @@
 ===Design Notes===
-This part was amplified from ''Bacillus subtilis'' genomic DNA and ligated into the ''EcoRI'' and ''PstI'' digested '''pSB1C3''' backbone.
+This part was amplified from ''<i>Bacillus subtilis</i>'' genomic DNA and ligated into the ''Eco''RI and ''Pst''I digested '''pSB1C3''' backbone.
 ===Source===
-This basic part consists of parts derived from PCR amplification of wild type ''B. subtilis'' chromosomal DNA (strain ''B. subtilis'' 168; Laboratory collection AG Stülke, Department for General Microbiology, University Göttingen).
+This basic part consists a gene that was amplified by PCR using chromosomal DNA of the wild type strain ''B. subtilis''  168 (Laboratory collection AG Commichau, Department for General Microbiology, University Göttingen, Germany).
 ===References===
+[https://www.ncbi.nlm.nih.gov/pubmed/18723616 (Zeigler <i>et al.</i> 2008)]: The origins of 168, W23, and other <i>Bacillus subtilis</i> legacy strains. J. Bacteriol. 190: 6983-95.