Difference between revisions of "Part:BBa K2832015:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | https://static.igem.org/mediawiki/parts/8/80/ApFAB82_Design_Diagram.png | |
| + | apFAB82 Test Device: The diagram specifies the design of the engineered E. coli apFAB82 Test Cells which are transformed by the plasmid pSB1C3-BBa_K2832015. The plasmid encodes the apFAB82 Test Device which contains the BIOFAB Promoter apFAB46. . This device was submitted for the silver medal requirement. | ||
| + | https://static.igem.org/mediawiki/parts/8/86/ApFAB82_Plasmid_Map.png | ||
| + | pSB1C3-BBa_K2832015 Plasmid Map: The plasmid contains apFAB82 Test Device | ||
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===References=== | ===References=== | ||
| + | Mutalik,V. Guimaraes, J. Cambray, G. Lam, C. Christoffersen, M. Mai, Q. Tran, A Morgan Paull, Keasling, J. Arkin, A. Endy, D. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements: Nature Methods, 354-360 | ||
Latest revision as of 20:01, 2 December 2018
BIOFAB Modular Promoter apFAB82 Driven mRFP1 Generator ( ---> mRFP1 )
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 630
Illegal AgeI site found at 742 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
apFAB82 Test Device: The diagram specifies the design of the engineered E. coli apFAB82 Test Cells which are transformed by the plasmid pSB1C3-BBa_K2832015. The plasmid encodes the apFAB82 Test Device which contains the BIOFAB Promoter apFAB46. . This device was submitted for the silver medal requirement.
pSB1C3-BBa_K2832015 Plasmid Map: The plasmid contains apFAB82 Test Device
Source
Source...
References
Mutalik,V. Guimaraes, J. Cambray, G. Lam, C. Christoffersen, M. Mai, Q. Tran, A Morgan Paull, Keasling, J. Arkin, A. Endy, D. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements: Nature Methods, 354-360