Difference between revisions of "Part:BBa K2832000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | https://static.igem.org/mediawiki/parts/9/9b/PbamE_design_diagram.png | |
| − | + | PbamE Design Diagram. The bamE promoter sequence binds Sigma Factor E (RpoE) when freed by the rpoE pathway. The pathway detects membrane damage via misfolded outer membrane proteins. mRFP1 signal indicates successful activation of the pathway. | |
| − | + | ||
| + | https://static.igem.org/mediawiki/parts/f/fb/PbamE_plasmid_map.png | ||
| + | FSU Promoter BBa_K2832000 inside plasmid pSB1C3. | ||
===Source=== | ===Source=== | ||
Latest revision as of 20:50, 1 December 2018
PbamE - Inducible Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 13
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PbamE Design Diagram. The bamE promoter sequence binds Sigma Factor E (RpoE) when freed by the rpoE pathway. The pathway detects membrane damage via misfolded outer membrane proteins. mRFP1 signal indicates successful activation of the pathway.
FSU Promoter BBa_K2832000 inside plasmid pSB1C3.
Source
E. coli K12 genome.