Difference between revisions of "Part:BBa K2533014:Design"

 
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===Design Notes===
 
===Design Notes===
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None.
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TH is transhydrogenase, gldA is glycerol dehydrogenase, lldP is the lactate channel. The whole circuit is synthesized by company.
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===Source===
 
===Source===
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===References===
 
===References===
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[1]Varman, A. M., Yu, Y., You, L., & Tang, Y. J. (2013). Photoautotrophic production of D-lactic acid in an engineered cyanobacterium. Microbial Cell Factories, 12, 117. http://doi.org/10.1186/1475-2859-12-117

Latest revision as of 14:57, 17 October 2018


RBS-TH-RBS-gldA-RBS-LLDP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 68
    Illegal NgoMIV site found at 569
    Illegal NgoMIV site found at 944
    Illegal NgoMIV site found at 1007
    Illegal NgoMIV site found at 1604
    Illegal NgoMIV site found at 2612
    Illegal NgoMIV site found at 2978
    Illegal AgeI site found at 1616
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2240


Design Notes

TH is transhydrogenase, gldA is glycerol dehydrogenase, lldP is the lactate channel. The whole circuit is synthesized by company.

Source

From Lactobacillus bulgaricus.


References

[1]Varman, A. M., Yu, Y., You, L., & Tang, Y. J. (2013). Photoautotrophic production of D-lactic acid in an engineered cyanobacterium. Microbial Cell Factories, 12, 117. http://doi.org/10.1186/1475-2859-12-117