Difference between revisions of "Part:BBa K2533003"
 
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ldhDARSdR can produce lactate, and lldP can release it from cytoplasm to the environment.
 
ldhDARSdR can produce lactate, and lldP can release it from cytoplasm to the environment.
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<h1>'''Characterization'''</h1>
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This is a two section for lactate produce and transfer part.
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[[File:T--HUST-China--2018-tonglu-ldhDARSdR-lldP.png ‎|400px|thumb|center|Figure1. ldhDARSdR-lldP]]
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<h2>DNA Gel Electrophoretic</h2>
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At first, we only have two simple genes as ldhDARSdR and lldP. And then we link them through overlap extension PCR. As the figure shows, we get the genetic stripe about 3000bp which means the overlap extension PCR is successful.
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[[File:T--HUST-China--2018-jiaotu-ldhDARSdR-lldP.jpeg|400px|thumb|center|Figure2. Overlap extension PCR of ldhDARSdR and lldP]]
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<h2>Metabolic figure</h2>
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[[File:T--HUST-China--2018-metabolic figure1.png|400px|thumb|center|Figure3. Metabolic figure of cyanobacteria with ldhDARSdR-lldP]]
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As the figure shows, with the help of light and PSI，Synechocystis sp. PCC 6803(cyanobacteria) with ldhDARSdR-lldP absorb CO<sub>2</sub> into Calvin cycle and produce pyruvate. And then ldhDARSdR can transfer pyruvate into lactate which will be transport to the environment by lldP.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:37, 17 October 2018


RBS-ldhDARSdR-RBS-lldP

ldhDARSdR can produce lactate, and lldP can release it from cytoplasm to the environment.

Characterization

This is a two section for lactate produce and transfer part.

Figure1. ldhDARSdR-lldP

DNA Gel Electrophoretic

At first, we only have two simple genes as ldhDARSdR and lldP. And then we link them through overlap extension PCR. As the figure shows, we get the genetic stripe about 3000bp which means the overlap extension PCR is successful.

Figure2. Overlap extension PCR of ldhDARSdR and lldP

Metabolic figure

Figure3. Metabolic figure of cyanobacteria with ldhDARSdR-lldP

As the figure shows, with the help of light and PSI，Synechocystis sp. PCC 6803(cyanobacteria) with ldhDARSdR-lldP absorb CO2 into Calvin cycle and produce pyruvate. And then ldhDARSdR can transfer pyruvate into lactate which will be transport to the environment by lldP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 780
    Illegal BglII site found at 1325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1461
    Illegal AgeI site found at 1924
    Illegal AgeI site found at 2362
    Illegal AgeI site found at 2521
    Illegal AgeI site found at 2533
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 764
    Illegal BsaI.rc site found at 1035