Difference between revisions of "Part:BBa I757006"
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<partinfo>BBa_I757006 short</partinfo> | <partinfo>BBa_I757006 short</partinfo> | ||
| − | * | + | * second fragment of the murine split enzyme dihydrofolatereductase (DHFR) |
| − | * purpose: split DHFR activity can be recovered | + | * purpose: split DHFR activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains (Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12141-6; Nat Biotechnol. 1999 Jul;17(7):683-90 ; J Mol Biol. 2000 Jan 21;295(3):627-39) |
* works with first half of split DHFR (part BBa_I757005) | * works with first half of split DHFR (part BBa_I757005) | ||
* NgoMIV / AgeI protein fusion part | * NgoMIV / AgeI protein fusion part | ||
Latest revision as of 15:51, 1 November 2007
DHFR2A_109-187_FusionPart
- second fragment of the murine split enzyme dihydrofolatereductase (DHFR)
- purpose: split DHFR activity can be recovered when halves are brought in close proximity by e.g. fusion to interacting domains (Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12141-6; Nat Biotechnol. 1999 Jul;17(7):683-90 ; J Mol Biol. 2000 Jan 21;295(3):627-39)
- works with first half of split DHFR (part BBa_I757005)
- NgoMIV / AgeI protein fusion part
- iGEM Team Freiburg 2007
- synthetic DNA by GeneArt
- Part in pGA4 vector (AmpR, ColEI ori)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 247 - 1000COMPATIBLE WITH RFC[1000]