Difference between revisions of "Part:BBa J100444:Experience"

 
 
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+
 
−
how you used this part and how it worked out.
+
<center>
 +
[[File:wed_rscar.png|500px]]
 +
</center>  
 +
 
 +
We grew colonies on three different Petri dishes over night in LB amp media. Each Petri dish tested a different part of the experiment. One was a positive control (labeled P) that would produce red colonies. Another was the negative control (labeled N) that would grow green colonies. The last Petri dish (labeled X) would contain our transformed plasmids containing our mutated gene sequence. Then we harvested three different colonies from the X dish, a colony from the P dish, and a colony from the N dish. These were grown overnight in a LB amp media solution. We then took triplicate samples from each test tube and measured the fluorescence and cell density of each.
 +
 
 +
 
 +
 
 +
 
  
 
===Applications of BBa_J100444===
 
===Applications of BBa_J100444===

Latest revision as of 20:08, 19 September 2018


Wed rscar.png

We grew colonies on three different Petri dishes over night in LB amp media. Each Petri dish tested a different part of the experiment. One was a positive control (labeled P) that would produce red colonies. Another was the negative control (labeled N) that would grow green colonies. The last Petri dish (labeled X) would contain our transformed plasmids containing our mutated gene sequence. Then we harvested three different colonies from the X dish, a colony from the P dish, and a colony from the N dish. These were grown overnight in a LB amp media solution. We then took triplicate samples from each test tube and measured the fluorescence and cell density of each.



Applications of BBa_J100444

User Reviews

UNIQad5e443c65f0b0f5-partinfo-00000000-QINU UNIQad5e443c65f0b0f5-partinfo-00000001-QINU