Difference between revisions of "Part:BBa I759002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | A pTet promoter (can be repressed by tet Repressor, and transcription activity can be restored by addition of anhydrotetracycline (aTc)) produces transcripts of a spacer (including the RBS)-cro complex. The spacer is a temporary placeholder, where later cis sequences (for cis riboregulator) will be cloned in. | + | A pTet promoter (can be repressed by tet Repressor, and transcription activity can be restored by addition of anhydrotetracycline (aTc)) produces transcripts of a spacer (including the RBS)-cro complex. The spacer is a temporary placeholder, where later cis sequences (for cis riboregulator) will be cloned in. This construct is an intermediate, as the terminator sequence for the transcript has not yet been cloned it. |
===Source=== | ===Source=== | ||
Latest revision as of 04:05, 27 October 2007
pTet-spacer-cro
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 174
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A pTet promoter (can be repressed by tet Repressor, and transcription activity can be restored by addition of anhydrotetracycline (aTc)) produces transcripts of a spacer (including the RBS)-cro complex. The spacer is a temporary placeholder, where later cis sequences (for cis riboregulator) will be cloned in. This construct is an intermediate, as the terminator sequence for the transcript has not yet been cloned it.
Source
Ptet was a standardized iGEM part, and the cro gene was from the lambda zap genome. Spacer-primers were specially designed so that the two parts (pTet and cro gene) could, through overlap extension PCR, be joined by the intermediary spacer.