Difference between revisions of "Part:BBa K2624004:Design"
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Please refer to the wiki of CPU_CHINA 2018 because this part is strictly related to the other parts used in the project.<br/> | Please refer to the wiki of CPU_CHINA 2018 because this part is strictly related to the other parts used in the project.<br/> | ||
The sequence of the inhibitory strand is dependent on the pri-miRNA basal segments that we target. Then we need to make sure that the actual appearance of the product of this part is indeed only the wanted sequence, with no 'tail'. So we added a hammerhead ribozyme in the 3'-end, and a RNA pol III promoter. | The sequence of the inhibitory strand is dependent on the pri-miRNA basal segments that we target. Then we need to make sure that the actual appearance of the product of this part is indeed only the wanted sequence, with no 'tail'. So we added a hammerhead ribozyme in the 3'-end, and a RNA pol III promoter. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 16:30, 26 August 2018
U6-inhSi-pri-miRNA
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 312
Illegal NgoMIV site found at 341 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 305
Design Notes
Please refer to the wiki of CPU_CHINA 2018 because this part is strictly related to the other parts used in the project.
The sequence of the inhibitory strand is dependent on the pri-miRNA basal segments that we target. Then we need to make sure that the actual appearance of the product of this part is indeed only the wanted sequence, with no 'tail'. So we added a hammerhead ribozyme in the 3'-end, and a RNA pol III promoter.
Source
The U6 promoter is from BBa_K2200003 and it is widely used, inside or outside the iGEM competition. The inhibitory strand is purly through our design. We did not refer to any public database.
References
[1]Dahlberg J E, Lund E. The genes and transcription of the major small nuclear RNAs[M]//Structure and function of major and minor small nuclear ribonucleoprotein particles. Springer, Berlin, Heidelberg, 1988: 38-70.
[2]Kao C C, Yang X, Kline A, et al. Template requirements for RNA synthesis by a recombinant hepatitis C virus RNA-dependent RNA polymerase[J]. Journal of virology, 2000, 74(23): 11121-11128.
[3]Han J, Lee Y, Yeom K H, et al. Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex[J]. Cell, 2006, 125(5): 887-901.