Difference between revisions of "Part:BBa K2668020:Design"

 
 
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===Design Notes===
 
===Design Notes===
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This part was supposed to be cloned from one gBlock but due to some synthesis issues from IDT we had to use multiple insert in fusion cloning.  
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The part BBa_K2668020 named Sirus comes from the <a href="http://2018.igem.org/Team:Toulouse-INSA-UPS/Design">iGEM team Toulouse-INSA-UPS 2018 Cerberus Project</a>. It was designed to investigate the binding capability of CBM3a to cellulose.  
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===Source===
 
===Source===
  
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These parts were recieved from IDT gBlock offer
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The part was obtained by synthesis from IDT. The CBM3a domain is a corrected version of the Part:BBa_K1321014. The mRFP1 is a corrected version of the Part:BBa_E1010.
  
 
===References===
 
===References===

Latest revision as of 15:11, 13 October 2018


Sirius: CBM3a - mRFP1 fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1272
    Illegal AgeI site found at 1384
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part BBa_K2668020 named Sirus comes from the iGEM team Toulouse-INSA-UPS 2018 Cerberus Project. It was designed to investigate the binding capability of CBM3a to cellulose.


Source

The part was obtained by synthesis from IDT. The CBM3a domain is a corrected version of the Part:BBa_K1321014. The mRFP1 is a corrected version of the Part:BBa_E1010.

References