Difference between revisions of "Part:BBa K2719007:Experience"
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===Applications of BBa_K2719007=== | ===Applications of BBa_K2719007=== | ||
| + | <p>This part was cloned on pSB1C3 using EcoRI and PstI, later it was transformed on <i>E.coli</i> DH5a (Figure 1).</p> | ||
| + | [[file:T--TecCEM--BBCol5Colonies.png|500px]] | ||
| + | <p><i>Figure 1.</i> Colonies transformed with Collagen V-like expression device, the red strain indicates a successful clonation and transformation of the plasmid</p> | ||
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| + | <p>Then the plasmid was extracted and run on an agarose gel electrophoresis (Figure 2). Later an restriction was performed using NotI and was run on an agarose gel (Figure 3) to probe the presence of the insert. Later the extracted plasmid was transformed on <i>E.coli</i> Rossetta (DE3).</p> | ||
| + | [[file:T--TecCEM--GelK2719007.png|500px]] | ||
| + | <p><i>Figure 2.</i> 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 9)Extraction Collagen V-like (BBa_K2719007), 10)Extraction Collagen V-like (BBa_K2719007) duplicate.</p> | ||
| + | [[file:T--TecCEM--GelK2719007R.png|500px]] | ||
| + | <p><i>Figure 3.</i> Collagen V-Like restriction with NotI in a 0.85% agarose gel with GelRed, MW) 2-Log DNA Ladder, 5)Collagen V-like (BBa_K2719007).</p> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 22:23, 17 October 2018
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Applications of BBa_K2719007
This part was cloned on pSB1C3 using EcoRI and PstI, later it was transformed on E.coli DH5a (Figure 1).
Figure 1. Colonies transformed with Collagen V-like expression device, the red strain indicates a successful clonation and transformation of the plasmid
Then the plasmid was extracted and run on an agarose gel electrophoresis (Figure 2). Later an restriction was performed using NotI and was run on an agarose gel (Figure 3) to probe the presence of the insert. Later the extracted plasmid was transformed on E.coli Rossetta (DE3).
Figure 2. 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 9)Extraction Collagen V-like (BBa_K2719007), 10)Extraction Collagen V-like (BBa_K2719007) duplicate.
Figure 3. Collagen V-Like restriction with NotI in a 0.85% agarose gel with GelRed, MW) 2-Log DNA Ladder, 5)Collagen V-like (BBa_K2719007).
User Reviews
UNIQ0683782e93b8fdb8-partinfo-00000000-QINU UNIQ0683782e93b8fdb8-partinfo-00000001-QINU