Difference between revisions of "Part:BBa K2719006:Experience"
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===Applications of BBa_K2719006=== | ===Applications of BBa_K2719006=== | ||
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| + | <p>This part was cloned on pSB1C3 using EcoRI and PstI and latter transformed into <i>E.coli</i> DH5a. The colonies obtained can be seen on figure 1.</p> | ||
| + | [[file:T--TecCEM--Col5Colonies.png|500px]] | ||
| + | <p><i>Figure 1.</i> Colonies transformed with Collagen V-like.</p> | ||
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| + | <p>Then the plasmid was extracted and run by duplicated on an agarose gel electrophoresis (Figure 2)</p> | ||
| + | [[file:T--TecCEM--Col5Extraction.png|500px]] | ||
| + | <p><i>Figure 2.</i> 0.85% agarose gel with GelRed: MW) 1Kb plus from NEB; 3) Collagen V- like (BBa_K2719006) V'; 4) Collagen V- like (BBa_K2719006) V''</p> | ||
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| + | <p>Latter it was digested using NotI to probe the presence of the insert, the restriction was also run on a agarose gel electrophoresis.</p> | ||
| + | [[file:T--TecCEM--ResK2719006.png|500px]] | ||
| + | <p><i>Figure 3.</i> 0.85% agarose gel with GelRed: MW) 2-Log DNA Ladder; 2) Collagen V- like (BBa_K2719006) restricted with NotI</p> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 22:19, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K2719006
This part was cloned on pSB1C3 using EcoRI and PstI and latter transformed into E.coli DH5a. The colonies obtained can be seen on figure 1.
Figure 1. Colonies transformed with Collagen V-like.
Then the plasmid was extracted and run by duplicated on an agarose gel electrophoresis (Figure 2)
Figure 2. 0.85% agarose gel with GelRed: MW) 1Kb plus from NEB; 3) Collagen V- like (BBa_K2719006) V'; 4) Collagen V- like (BBa_K2719006) V
Latter it was digested using NotI to probe the presence of the insert, the restriction was also run on a agarose gel electrophoresis.
Figure 3. 0.85% agarose gel with GelRed: MW) 2-Log DNA Ladder; 2) Collagen V- like (BBa_K2719006) restricted with NotI
User Reviews
UNIQ0d64224be3f0b492-partinfo-00000000-QINU UNIQ0d64224be3f0b492-partinfo-00000001-QINU