Difference between revisions of "Part:BBa K2719005"

Latest revision as of 13:00, 17 October 2018

Tenascin Domain V Expression Device

Tenascin was modified and optimized for Escherichia coli BL21-DE3 in order to be detected and processed by this strain. In addition, a GTS protein was fusionated to tenascine by a polylinker to conserve the three dimensional structure, because GST provides stability to the Tenascin domain and, also, is a protein that was useful for purification in the case HIS tag doesn’t worked well. This part contain a T7-lac promoter (BBa_R0184), and RBS (BBa_J611000) and a rrnB-T1 terminator (BBa_B0015) using RFC10 assembly standard, thus containing the standard BioBrick prefix and suffix.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 416

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2719005 short</partinfo>
-Tenascin Domain V expression Device.
+Tenascin was modified and optimized for <i>Escherichia coli</i> BL21-DE3 in order to be detected and processed by this strain. In addition, a GTS protein was fusionated to tenascine by a polylinker to conserve the three dimensional structure, because GST provides stability to the Tenascin domain and, also, is a protein that was useful for purification in the case HIS tag doesn’t worked well. This part contain a T7-lac promoter (BBa_R0184), and RBS (BBa_J611000) and a rrnB-T1 terminator (BBa_B0015) using RFC10 assembly standard, thus containing the standard BioBrick prefix and suffix.
 <!-- Add more about the biology of this part here