Difference between revisions of "Part:BBa K2719004:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K2719004 short</partinfo>
 
<partinfo>BBa_K2719004 short</partinfo>
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<p>This is a quaternary protein with the five domains that composed the complete tenascin protein. It has been optimized for Escherichia coli DH5a (DE3) and cloned in a pSB1C3 plasmid. It is also a protein mainly composed by beta-pleated sheets and several Random Coils, giving to the domains mobility. (Figure 1) </p>
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"https://static.igem.org/mediawiki/2018/1/10/T--TecCEM--TCD5%2BGST3DStructure.png"
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<p><i>Figure 1.</i> Tenascin V Fibronectin Domains 3D Structure. Modelled with Swiss-Model.</p>
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<partinfo>BBa_K2719004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2719004 SequenceAndFeatures</partinfo>
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===References===
 
===References===
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RChiqut, et. al.(1991). Tenascin variants: differential binding to fibronectin and distinct distribution in cell cultures and tissues. doi:  10.1091/mbc.2.11.927

Latest revision as of 03:17, 18 October 2018


Tenascin Fibronectin Domains (I-V)

This is a quaternary protein with the five domains that composed the complete tenascin protein. It has been optimized for Escherichia coli DH5a (DE3) and cloned in a pSB1C3 plasmid. It is also a protein mainly composed by beta-pleated sheets and several Random Coils, giving to the domains mobility. (Figure 1)

"T--TecCEM--TCD5%2BGST3DStructure.png"

Figure 1. Tenascin V Fibronectin Domains 3D Structure. Modelled with Swiss-Model.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized to E.coli BL21


Source

Genomic library.

References

RChiqut, et. al.(1991). Tenascin variants: differential binding to fibronectin and distinct distribution in cell cultures and tissues. doi: 10.1091/mbc.2.11.927