Difference between revisions of "Part:BBa K2719002:Experience"
| (4 intermediate revisions by 2 users not shown) | |||
| Line 5: | Line 5: | ||
===Applications of BBa_K2719002=== | ===Applications of BBa_K2719002=== | ||
| + | <p>To confirm the presence of Tenascin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in <i>Escherichia coli</i> DH5a. (Figure 2)</p> | ||
| + | [[file:T--TecCEM--TCD5Colonies.png|500px]] | ||
| + | <p><i>Figure 1.</i> Transformed Tenascin 5 Domain V colonies</p> | ||
| + | <p>To prove the presence of the plasmid in <i>E. coli</i> it was necessary to create an agarose gel. Because polyproline linker wasn’t binded in this fusion protein, the bands presented in the gel were less heavy. (Figure 2). </p> | ||
| + | <p>After that, another agarose gel was made for the restriction parts to confirm the presence of the insert (figure 3).</p> | ||
| + | |||
| + | [[file:T--TecCEM--FusGCT.png|500px]] | ||
| + | <p><i>Figure 2.</i>GST and Tenascin V Fusion agarose gel at 0.85% with GelRed: MW) 1Kb plus from NEB; 3) GST and Tenascin V Fusion (BBa_K2719002) V'; 4) GST and Tenascin V Fusion (BBa_K2719002) V''</p> | ||
| + | |||
| + | [[file:T--TecCEM--ResK2719002.png|500px]] | ||
| + | <p><i>Figure 3.</i>GST and Tenascin V Fusion restriction with NotI agarose gel at 0.85% with GelRed: MW) 1Kb plus from NEB; 5) GST and Tenascin V Fusion (BBa_K2719002) V'; 7) GST and Tenascin V Fusion (BBa_K2719002) V''</p> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 21:21, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2719002
To confirm the presence of Tenascin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 2)
Figure 1. Transformed Tenascin 5 Domain V colonies
To prove the presence of the plasmid in E. coli it was necessary to create an agarose gel. Because polyproline linker wasn’t binded in this fusion protein, the bands presented in the gel were less heavy. (Figure 2).
After that, another agarose gel was made for the restriction parts to confirm the presence of the insert (figure 3).
Figure 2.GST and Tenascin V Fusion agarose gel at 0.85% with GelRed: MW) 1Kb plus from NEB; 3) GST and Tenascin V Fusion (BBa_K2719002) V'; 4) GST and Tenascin V Fusion (BBa_K2719002) V
Figure 3.GST and Tenascin V Fusion restriction with NotI agarose gel at 0.85% with GelRed: MW) 1Kb plus from NEB; 5) GST and Tenascin V Fusion (BBa_K2719002) V'; 7) GST and Tenascin V Fusion (BBa_K2719002) V
User Reviews
UNIQf63bd17fbd445401-partinfo-00000000-QINU UNIQf63bd17fbd445401-partinfo-00000001-QINU