Difference between revisions of "Part:BBa J100430"

 
 
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<partinfo>BBa_J100430 short</partinfo>
 
<partinfo>BBa_J100430 short</partinfo>
  
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Gal4 binding site inserted downstream of a T7 promoter consensus sequence [https://parts.igem.org/wiki/index.php?title=Part:BBa_J100403 (J100403)] that will produce RFP when inserted into pClone Red [https://parts.igem.org/Part:BBa_J100397 (J119137)]. When present the Gal4 protein will bind to the site downstream of the promoter.
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Gal4 binding site inserted downstream of a T7 promoter consensus sequence [https://parts.igem.org/wiki/index.php?title=Part:BBa_J100403 (J100403)] that will produce mScarlet when inserted into pClone mScarlet [https://parts.igem.org/Part:BBa_J100397 (J119137)]. When present the Gal4 protein will bind to the site downstream of the promoter.
  
 
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Latest revision as of 20:36, 29 June 2018


T7 Promoter with Downstream Gal4 Binding Site in pClone mScarlet

Gal4 binding site inserted downstream of a T7 promoter consensus sequence (J100403) that will produce mScarlet when inserted into pClone mScarlet (J119137). When present the Gal4 protein will bind to the site downstream of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 912
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 912
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 912
    Illegal BamHI site found at 858
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 912
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 912
  • 1000
    COMPATIBLE WITH RFC[1000]