Difference between revisions of "Part:BBa J100414"
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<partinfo>BBa_J100414 short</partinfo> | <partinfo>BBa_J100414 short</partinfo> | ||
| − | Gal4 binding site inserted downstream of a T7 promoter consensus sequence [https://parts.igem.org/wiki/index.php?title=Part:BBa_J100403 (J100403)] that will produce RFP when inserted | + | Gal4 binding site inserted downstream of a T7 promoter consensus sequence [https://parts.igem.org/wiki/index.php?title=Part:BBa_J100403 (J100403)] that will produce RFP when inserted into pClone Red [https://parts.igem.org/Part:BBa_J119137 (J119137)] . When present the Gal4 protein will bind to the site downstream of the promoter. |
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Latest revision as of 14:17, 26 June 2018
T7 Promoter with Downstream Gal4 Binding Site in pClone Red
Gal4 binding site inserted downstream of a T7 promoter consensus sequence (J100403) that will produce RFP when inserted into pClone Red (J119137) . When present the Gal4 protein will bind to the site downstream of the promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1454
Illegal AgeI site found at 1566 - 1000COMPATIBLE WITH RFC[1000]