Difference between revisions of "Part:BBa K2876005:Design"

 
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===Design Notes===
 
===Design Notes===
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This part needed a 3-Alanine linker
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The part needed a 3-Alanine linker. It was codon optimized for E. coli.
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===Source===
 
===Source===
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===References===
 
===References===
 +
Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0
 +
 +
Li, X., Li, P. and Zhang, Q., Molecular analysis of monoclonal antibodies against aflatoxins: a prediction of functional amino acid which influences antibody affinity. Chinese Academy of Agricultural Sciences, 2011.
 +
 +
Pei, S.a.S., D., Construction and identification of mouse Anti-AFB1 phage single-chain antibody library. Food and Biological Engineering College, 2010.

Latest revision as of 07:14, 17 October 2018


A fusion of a RNAPolIII alpha subunit and antiAFB1-ScFv1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 8
    Illegal NotI site found at 766
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 480
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part needed a 3-Alanine linker. It was codon optimized for E. coli.

Source

Part:BBa_K2247007, BBa_K2876002, BBa_K2876004

References

Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0

Li, X., Li, P. and Zhang, Q., Molecular analysis of monoclonal antibodies against aflatoxins: a prediction of functional amino acid which influences antibody affinity. Chinese Academy of Agricultural Sciences, 2011.

Pei, S.a.S., D., Construction and identification of mouse Anti-AFB1 phage single-chain antibody library. Food and Biological Engineering College, 2010.