Difference between revisions of "Part:BBa K2876000:Design"

 
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===Design Notes===
 
===Design Notes===
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This promoter has been optimized for E. coli
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This promoter has been optimized for E. coli, and has been modified from a traditional pLacZ promoter to allow upstream lambda-cl bonding.
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===Source===
 
===Source===
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===References===
 
===References===
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Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0

Latest revision as of 06:50, 17 October 2018


placOL2-62 E. coli promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 25
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 25
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 25
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 25
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter has been optimized for E. coli, and has been modified from a traditional pLacZ promoter to allow upstream lambda-cl bonding.

Source

Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0

References

Dove, S. L., Joung, J. K., & Hochschild, A. (1997). Activation of prokaryotic transcription through arbitrary protein- protein contacts. Nature. http://doi.org/10.1038/386627a0