Difference between revisions of "Part:BBa J100406"

Latest revision as of 20:54, 22 June 2018

Strong T7 Promoter (R0085) Followed by Strong C-Dog (J119028)

This is a combination of the Strong T7 Promoter BBa_R0085 and the Strong C-Dog Ribosome Binding Site BBa_J119028. We designed this part in order to test our mechanism for in vitro artificial selection using the SmaI enzyme. We changed the first G to a C in the C-Dog to remove the ApaI site there. We also removed the final ATG from the end of the C-dog because we already had one at the start of the SmaI gene we are using.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_J100406 short</partinfo>
-This is a combination of the Strong T7 Promoter <https://parts.igem.org/Part:BBa_R0085 BBa_R0085> and the Strong C-Dog Ribosome Binding Site BBa_J119028. We designed this part in order to test our mechanism for in vitro artificial selection using the SmaI enzyme. We changed the first G to a C in the C-Dog to remove the ApaI site there. We also removed the final ATG from the end of the C-dog because we already had one at the start of the SmaI gene we are using.
+This is a combination of the Strong T7 Promoter [https://parts.igem.org/Part:BBa_R0085 BBa_R0085] and the Strong C-Dog Ribosome Binding Site [https://parts.igem.org/Part:BBa_J119028 BBa_J119028]. We designed this part in order to test our mechanism for in vitro artificial selection using the SmaI enzyme. We changed the first G to a C in the C-Dog to remove the ApaI site there. We also removed the final ATG from the end of the C-dog because we already had one at the start of the SmaI gene we are using.
 <!-- Add more about the biology of this part here