Difference between revisions of "Part:BBa M50439:Experience"
(→Stanford Location) |
|||
| Line 26: | Line 26: | ||
Plasmid name: pHlash<BR> | Plasmid name: pHlash<BR> | ||
| − | DNA2.0 Gene #: | + | DNA2.0 Gene #: pD1204-NSH <BR> |
Organism: S. cerevisiae <BR> | Organism: S. cerevisiae <BR> | ||
Device type: Sensor<BR> | Device type: Sensor<BR> | ||
Glycerol stock barcode: 133021752<BR> | Glycerol stock barcode: 133021752<BR> | ||
Box label: BioE44 S18 | Box label: BioE44 S18 | ||
Latest revision as of 11:58, 12 June 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_M50439
This construct can successfully be used to measure intracellular pH given by a BRET ratio in S. cerevisiae. Due to the well-characterized pH-sensitivity of Rluc8 (luciferase), we are confident that BRET readout from cpVenus (fluorophore) is indicative of intracellular pH because it was excited through Rluc8 activity.
User Reviews
UNIQ87d1bad345881232-partinfo-00000000-QINU UNIQ87d1bad345881232-partinfo-00000001-QINU
This plasmid was used in Bba_M50440 and the user experience is better outlined there.
Stanford Location
Plasmid name: pHlash
DNA2.0 Gene #: pD1204-NSH
Organism: S. cerevisiae
Device type: Sensor
Glycerol stock barcode: 133021752
Box label: BioE44 S18